美能注射液治疗玫瑰糠疹48例临床观察

目的观察美能注射液治疗玫瑰糠疹的临床疗效。方法80例患者被随机分为治疗组48例和对照组32例,对照组口服氯雷他定、维乐生片、复方蒲公英片治疗,治疗组在此治疗的基础上应用美能注射液40 mL/d静脉滴注。结果治疗组总有效率为77.1%,对照组总有效率为53.1%,两组比较差异有显著性(P<0.05)。结论美能治疗玫瑰糠疹疗效较好,值得临床推广应用。     

玫瑰糠疹皮损血管细胞黏附分子-1的检测

目的探讨血管细胞黏附分子-1(VCAM-1)在玫瑰糠疹中的发病机制。方法采用免疫组化SP法检测玫瑰糠疹皮损血管内皮细胞VCAM-1的表达。结果皮损组血管内皮细胞VCAM-1的表达均高于正常皮肤组及玫瑰糠疹皮损周围正常皮肤组,玫瑰糠疹皮损周围正常皮肤组的表达高于正常皮肤组,差异均有显著性(P<0.05)。结论VCAM-1在玫瑰糠疹皮损血管内皮细胞上的高表达与真皮浅层血管周围淋巴细胞浸润有关,参与了玫瑰糠疹的炎症反应。     

玫瑰糠疹与柯萨奇B组病毒感染关系的研究

用病毒学及免疫学方法研究了玫瑰糠疹与柯萨奇Ｂ组病毒感染的关系。结果表明，急性期患者血清抗柯萨奇Ｂ组病毒特异性ＩｇＭ的阳性率明显高于健康对照组，血清循环免疫复合物的含量急性期明显高于恢复期，中和试验和病毒分离都证明患者体内有柯萨奇Ｂ组病毒感染。这些结果提示，玫瑰糠疹的发病与柯萨奇Ｂ组病毒感染有直接关系，而造成免疫病理损伤的原因是柯萨奇Ｂ组病毒与其相应抗体形成的循环免疫复合物。     

Epidemiological study of human herpesvirus-6 and human herpesvirus-7 in pityriasis rosea

BACKGROUND: Pityriasis rosea (PR) is a common papulosquamous skin disorder that is suspected to have an infectious aetiology. OBJECTIVES: We aimed to study the role of human herpesvirus (HHV)-7 and HHV-6 in the pathogenesis of PR. METHODS: We performed seroepidemiological studies (indirect immunofluorescence test) and polymerase chain reaction (PCR) analysis for HHV-6 and HHV-7 in patients with PR. Seventy-two serum samples and 37 samples of peripheral blood mononuclear cells (PBMC) from 44 patients with PR were obtained. Twenty-five patients with other skin disorders such as drug eruption, urticaria or herpes zoster were studied as controls in the PCR analysis. RESULTS: HHV-7 DNA was detected in 13 of 30 (43%) samples of PBMC of the patients with PR and 14 of 25 (56%) samples of PBMC of controls. HHV-6 DNA was detected in six of 29 (21%) patients with PR and nine of 23 (39%) controls. Thus there was no difference in the prevalence of HHV-6 or HHV-7 in PBMC between patients with PR and those with other skin disorders. In the seroepidemiological study, two cases of at least a fourfold rise in titre and five cases of a fourfold decrease in titre to HHV-7 antibody, and two cases of a fourfold rise in titre and two cases of a fourfold decrease in titre to HHV-6 antibody, were observed in 24 patients with PR. This seroepidemiological study revealed antibody responses consistent with active infection in several PR patients, but the greater proportion of the patients had no definite increase in the antibody titres. CONCLUSIONS: We conclude that HHV-7 and HHV-6 may play a part in some patients with PR, but that other causative agents may exist. Further analyses are needed to determine the causative agents of PR.     

红景天对睡眠剥夺大鼠学习记忆的影响及其机制探索

目的:探讨红景天对睡眠剥夺大鼠学习记忆功能的影响及其可能机制。方法:用小平台水环境法建立睡眠剥夺模型,每组半数行Y型电迷宫测试,并检测海马和额叶组织中超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量,半数利用免疫组化法检测海马区胆碱乙酰转移酶(ChAT)及胶质纤维酸性蛋白(GFAP)表达。结果:Y型电迷宫测试显示,与模型组相比,红景天组全天总反应时间(TRT)和错误反应次数(EN)均较低(P<0.05)。与模型组相比,红景天组海马组织和额叶组织SOD活力均较高(P<0.05)。与模型组相比,红景天组海马组织MDA含量较低(P<0.05),额叶组织MDA含量与模型组差别无统计学意义(P>0.05)。ChAT表达检测表明,与模型组相比,红景天组阳性细胞着色较深,轮廓较清晰,排列较整齐,各亚区阳性细胞数量较多(P<0.05)。GFAP表达检测显示,与模型组相比,红景天组阳性细胞着色较浅,细胞体积较小,突起较短较细,侧枝较少,阳性细胞数量较少(P<0.05)。结论:红景天对睡眠剥夺后大鼠脑组织氧化损伤及神经元损伤有保护作用,可缓解睡眠剥夺造成的学习记忆功能的下降。     

Analgesic and Antipyretic Activities of n-Butanol Alkaloids Extracted from the Stem Bark Hunteria Zeylanica and its Major Constituent,Strictosidinic Acid,in Mice

The pharmacological activities of the n -butanol alkaloids extracted from the stem bark of Hunteria zeylanica (Retz) Gardn. ex Thw. ( H. zeylanica ) and its major constituent, strictosidinic acid, on nociceptive response using writhing and hot plate tests, the antipyretic activity in yeast-induced fever, pentobarbital-induced sleep, and locomotor activity were examined in mice. Oral administration of H. zeylanica extract at 200 mg/kg significantly decreased the number of contortions and stretchings induced by acetic acid but not heat-induced pain. Strictosidinic acid (5–20 mg/kg, p.o.) also produced a similar effect but less pronounced than the extract. The antipyretic effect of strictosidinic acid (5–20 mg/kg, p.o.) was stronger than that of the extract (100–200 mg/kg, p.o.). The H. zeylanica extract dose-dependently (50–200 mg/kg, p.o.) prolonged the duration of pen-tobarbital-induced sleep but had no sign ificant effect on locomotor activity. No effect of strictosidinic acid was noted on both pentobarbital-induced sleep and locomotor activity. These results suggest that the H. zeylanica extract possesses peripheral analgesic and mild antipyretic effects and its major constituent, strictosidinic acid, exerts a similar analgesic effect with marked antipyretic activity.     

维药蜀葵花化学成分的分离与结构鉴定(Ⅰ)

目的扩大维药蜀葵花(Althaea rosea(Linn.)Cavan.)的药用资源。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱手段对蜀葵花进行分离纯化,并通过理化性质与波谱分析方法鉴定化合物的结构。结果从蜀葵花体积分数95%乙醇提取物中分离得到14个单体成分,分别鉴定为东莨菪内酯(scopoletin,1)、3,5,7-三羟基色原酮(3,5,7-trihydroxychromone,2)、反式咖啡酸(trans-caffeic acid,3)、4-hydroxy-3-methoxycinnamyl-β-D-glucopyranoside(4)、3,4-dime-thoxycinnamyl-β-D-glucopyranoside(5)、对羟基苯甲醛(p-hydroxybenzaldehyde,6)、对羟基苯甲酸(p-hydroxybenzoic acid,7)、4-O-β-D-吡喃葡萄糖氧基苯甲酸(4-O-β-D-glucopyranosylbenzoate,8)、对羟基苯甲酸-β-D-吡喃葡萄糖酯苷(p-hydroxybenzoyl-β-D-glucopyranoside,9)、4-methoxybenzyl-β-D-glucopyranoside(10)、原儿茶酸(protocatechuic acid,11)、3,5-二甲氧基-4-羟基苯甲酸(3,5-di-methoxy-4-hydroxy benzoic acid,12)、腺苷(adenosine,13)和1H-吲哚-3-羧酸(1H-indole-3-carboxylic acid,14)。结论化合物2、4～6、8～10和14为首次从蜀葵属中分离得到,3、11和13为首次从蜀葵中分离得到,并首次报道了化合物5的核磁共振波谱数据。     

地塞米松、沙丁胺醇气雾剂联合红景天治疗100例急性高原反应的临床观察

目的:观察地塞米松、沙丁胺醇气雾剂联合红景天治疗急性高原反应的临床疗效。方法:选择急性高原反应患者100例作为观察对象,按照随机数字表法分为观察组和对照组,每组50例。观察组采用地塞米松、沙丁胺醇气雾剂联合红景天口服液进行治疗。对照组采用红景天口服液进行治疗。在用药15 d后测定患者呼吸频率、脉搏、血压、红细胞计数及血红蛋白水平等指标,比较2组疗效。结果:观察组总有效率高于对照组(84.0%vs 62.0%,P0.05)。用药后2组高原反应基础生理指标中呼吸频率、收缩压及观察组脉搏、舒张压、红细胞计数及血红蛋白水平均显著降低(P0.05或P0.01),且观察组上述指标均显著低于对照组(均P0.05或P0.01);观察组患者的急性高原反应评分明显低于对照组,血氧饱和度明显高于对照组(均P0.01)。结论:地塞米松、沙丁胺醇气雾剂联合红景天能够有效控制患者的急性高原反应症状,改善患者的高原反应基础生理指标,降低患者的急性高原反应评分,缩短病程。     

重组海葵溶细胞素及平颏海蛇磷脂酶A2对血管外膜成纤维细胞增殖的影响

目的 观察重组海葵溶细胞素(rSrc)及平颏海蛇磷脂酶A₂(rPLA₂)对成纤维细胞增殖的影响。方法 体外培养成纤维细胞,应用不同浓度的rSrc和rPLA₂分别进行作用并设立对照进行比较,采用非放射性的MTS/PES法确定成纤维细胞的增殖状态。结果 各组细胞增殖率分别为:对照组0.840±0.061、rSrc 100 μg/ml组0.263±0.044、rSrc 10 μg/ml组0.418±0.054、rSrc 1 μg/ml组0.605±0.063、rSrc 100 ng/ml组0.772±0.054、rSrc 10 ng/ml组0.906±0.072、rPLA₂ 100 μg/ml组0.498±0.076、rPLA₂ 10 μg/ml组0.937±0.112、rPLA₂ 1 μg/ml组0.978±0.145。统计分析显示,低至1 μg/ml的rSrc仍能明显抑制大鼠成纤维细胞的增殖(P<0.01),并呈现剂量依赖性(P<0.05)。rPLA₂ 100 μg/ml组与对照组相比细胞增殖率有显著差异(P<0.05);rPLA₂ 10 μg/ml和rPLA₂ 1 μg/ml组与对照组相比均无显著差异(P>0.05)。结论 rSrc和rPLA₂分别对血管外膜成纤维细胞增殖有一定的抑制作用。     

The sooth beneath the taste roseas in the urethra and first description of neuro-morpho-chemical mechanism of penile erectile posture in males: an experimental study

Aim: The morphologic mechanism of orgasmic sensation has not yet been understood. Taste roseas may be stimulated by fructose via pudendal nerves, which has not been studied yet.

Methods: In this study, 27 male adult rabbits were used, which were divided into three groups: 5 as control; 5 as SHAM and 17 used as study group. We injected 0.2 cc of distilled water to SHAM and 0.2 cc of fructose solution to the study group of their urethral orifices, and examined the occurrence of penile erection. The relationship between erection and pudendal nerve ganglia and penile tissues was statistically compared.

Results: In animals with high neuron density of pudendal ganglia, more erection phenomenon was observed than those animals with low neuron density. Interestingly, neuron density of pudendal ganglia was 9.243 ± 542 /mm³ in hypoactive and was 5.980 ± 463 /mm³ in non-active animals (p < 0.05).

Conclusions: The seminal fructose may stimulate taste roseas of the urethra and glans penis via pudendal nerves. The present study describes a new neuro-morpho-chemical mechanism of orgasmic sensation with its neurosurgical aspect.